NASA’s Artemis program in collaboration with European, Canadian, and Japanese space agencies, and various commercial partners, marks the return of humankind to the Moon. Unlike the Apollo lunar missions from fifty years ago, Artemis will set the stage for the continuous presence of humans on the Moon and pave a path for the journey to Mars and beyond. Plants will be an essential component of habitation systems that will enable humans to thrive on both the lunar and Mars surface, as well as space transit vehicles because of their nutritional and psychological benefits. Furthermore, plants will be valued in environmental control and life support systems because they generate oxygen, remove carbon dioxide, and recycle water and waste. However, an in-depth understanding of how plants and their associated microbial communities adapt to the harsh and confined environment of deep space is essential before they can be effectively used for long-duration space missions. This plenary symposium will address challenges and present potential solutions to growing plants in space. The four speakers will cover a broad range of topics, including mechanistic studies on fern resurgence after asteroid-triggered mass extinction events, artificial photosynthesis for space agriculture, and how the plant microbiome and an understanding of plant-pathogen dynamics in microgravity can guide crop production strategies in space.